The Rodolfus Choir is made up of singers aged 25 and younger who have been chosen from past and present members of the Eton College Choral Courses for prospective choral scholars. Each year these six week-long summer courses attract some 350 students between the ages of 16 and 20, and of these perhaps a dozen are invited to join the Rodolfus Choir as places become vacant. Many of these singers are choral scholars, some are at music college, and most hope to make a career in music.

Since its foundation in 1984 the choir has toured in France, Italy, and Austria, and has appeared at many English festivals including two appearances at the Three Choirs Festival in Gloucester. It has also performed throughout the United Kingdom at venues great and small, including St John's, Smith Square, where the choir has performed the Bach B-minor Mass with The Hanover Band and later with the Orchestra of the Age of Enlightenment, broadcast on Classic FM. In the last two years the choir has been more active than ever, singing in several festivals (BBC Proms, Ryedale, Three Choirs, Snape Proms and the York Early Music Festival), making three CDs (one for Naxos and two for Signum) and recording Songs of Praise, as well as performing the usual series of concerts. The choir is well-known for its imaginative programmes, and for its presentation of new music. Its recent CD recordings include music as diverse as Monteverdi, Grier, Tallis and German Romantic motets. All have attracted extremely complimentary press notices, with tracks broadcast by Brian Kay on his Sunday morning programme and also many on Classic FM and BBC Radio 3.

The choir's future engagements include a touring Duruflé Requiem in the UK, and participation in the festival Night of the Choirs in Belgium.